Nyias txheej chromatography: txoj kev yooj yim ntawm txoj kev tsom xam

Nyias txheej chromatography yuav siv qhov chaw hauv cov teeb meem ntawm ntau thiab ib qho kev ntsuas ntawm cov khoom siv tshuaj, khoom siv, kev kho mob-lom, technology, tshuaj lom neeg thiab ntau lwm yam. Cov txheej txheem nyias nyias chromatography tseem yog ib txoj kev tuaj yeem siv tau los ntawm txhua qhov chaw ntawm cov tshuaj.

Qhov no cov txheej txheem ntawm kev sib cais thiab tsom xam ntawm multi-tivthaiv systems yog lug siv nyob rau hauv lub chaw soj nstuam ntawm sib txawv heev tseem thiab cov kev taw qhia: tsis muaj kab mob-epidemiological, kho tsiaj, agrochemical, kev tshawb fawb, forensic, thiab kev cog kev tiv thaiv noj, Center rau standardization thiab lub Bureau of Forensic tshuaj.

Qhov tseem ceeb ntawm cov txheej txheem nyias nyias chromatography raws li lub zog ntawm cov tshuab (cov hnyav ntawm qhov analyte) hauv cov lus nug ntawm lub npe hu ua capillary rog yog qhov yooj yim thiab yooj yim ntawm cov txheej txheem chromatographic, cov qib siab ntawm qhov tseeb ntawm qhov kev ntsuam xyuas thiab tus nqi pheej yig ntawm cov cuab yeej tsim nyog.

Nyias txheej chromatography, muaj kev sib tw siab heev (qhov tsis tshua muaj neeg pom) thiab kev xaiv, tso cai rau kev txiav txim siab txog 10-20 μg ntawm cov tshuaj uas muaj tseeb ntawm 7%, uas yog qhov ntsuas kub siab heev. Nyias txheej chromatography yuav ua tau ib qho adsorption thiab kev faib tawm. Qhov kev xaiv thawj yog feem ntau siv dav.

Tus essence ntawm nyias txheej chromatography yog lub zog ntawm qhov hnyav hauv cov lus nug thiab qhov chaw faib ntau yam, nyob ntawm lawv tus kheej cov yam ntxwv thiab cov khoom. Qhov tseeb yog tias cov lwg me me ntawm qhov txawv txav thiab cov qauv yog qhov xa (qhov tso tawm) rau ntawm cov tawv nqaij ntawm chromatographic phaj hauv ntau txoj kev. Yog li, txoj hauv kev sib txuas ua ke yeej maj mam muab faib ua cov khoom sib txuas.

Tsiv ntawm kev sib txawv raws cov lus nug txheej txheem, cais, twb sib cais, yam khoom tsim ib chromatogram. Txhua qhov xim nqaij ntawd sib raug rau qee yam tshuaj yeeb dej caw. Cov khoom tsis muaj xim muaj teeb ci nrog lub teeb ci ultraviolet, nyob rau hauv cov kab uas muaj ntau lub tebchaw pib fluoresce, los yog raug kho nrog ib qho tshwj xeeb coloring reagent.

Cov niaj hnub analytical txoj kev GLC stands. Hom no ntawm kev sib cais thiab kev ntsuam xyuas yog cov ua cim tshwj xeeb uas tsis siv lub cev ua kua uas yog siv cov khoom nug, uas yog siv cov khoom ua kom zoo nkauj. Thiab raws li cov tsav tsheb nruab nrab siv no txawv inert gases. Hom no zoo tshaj plaws rau txoj kev kawm txog kev sib tov, uas muaj pua pua yam sib txawv.

Lwm niaj hnub txoj kev soj nyob tus yeees ntawm complex tshuaj yog kua chromatography. Tus qauv tseem ceeb ntawm tus qauv no yog kev sib cais ntawm cov khoom ib leeg los ntawm kev siv qhov sib txawv ntawm lawv qhov kev faib tawm ntawm lub xov tooj (eluent) thiab cov lus tsis txaus siab (nug). Hom no feem ntau yog siv los ntawm kev ntsuam xyuas ntawm ntau hom organic compounds (khoom noj khoom haus additives, tshuaj tua kab, co toxins ntau, thiab lwm yam). Tsis tas li no txoj kev ntawm kev tshawb nrhiav chromatographic yog siv los tswj cov tshuaj pharmacological preparations, los txiav txim seb cov ntsiab lus ntawm kev tso kua roj ntsha, cov tshuaj amino acid thiab lwm yam.